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Advanced Semen Analysis with sperm DNA fragmentation testing


Get deeper insights into your sperm health, from the comfort of home. Legacy’s Advanced Semen Analysis evaluates sperm count, motility, morphology, and sperm DNA fragmentation, a measure of sperm’s genetic health that can help shine a light on infertility, miscarriage, or failed IVF treatment. Option to add a sperm freezing plan.

Advanced Semen Analysis clients have access to:

    Free overnight shipping
    Expedited results available in 24–48 hours
    1:1 client support from Legacy fertility advisors
    Post-thaw analysis to assess whether you’re a good candidate for sperm freezing
HSA / FSA accepted


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+ Yearly



as low as $50/month

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Results in 48 hours

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Free shipping

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CLIA-certified lab

The Advanced Semen Analysis evaluates all key parameters of sperm quality:

Semen volume: How much semen you’re producing

Sperm concentration: How many sperm are present in your semen

Sperm motility: How many of your sperm are moving — a crucial indicator of your sperm’s health and fertility

Total motile sperm count: The final calculation of your sperm quantity and quality, which is highly correlated to your chances of creating a pregnancy

Sperm morphology: How many of your sperm are the proper size and shape

Sperm DNA fragmentation: The degree of damage to the genetic information carried by sperm, which can cause infertility, pregnancy loss, or health issues for the future child

Post-thaw analysis: We freeze and thaw a small portion of your sample to assess if it’s a good candidate for cryopreservation (sperm freezing)

We’re the only sperm DNA fragmentation testing option that uses an easy mail-in collection kit.

Legacy’s Advanced Semen Analysis looks at all the key metrics of sperm health, including sperm DNA fragmentation. Many at-home sperm testing options don’t examine sperm motility or morphology, which are important parameters of reproductive health.

Our test is a clinic-grade semen analysis — without the clinic.

The Advanced Semen Analysis with sperm DNA fragmentation testing is recommended for:

Those planning or undergoing fertility treatment.
In about 15% of cases, those with high sperm DNA fragmentation will have normal parameters on a routine semen analysis. Exploring sperm’s genetic health can offer an explanation as to otherwise unexplained infertility, or can help your healthcare team understand why a fertility treatment cycle has failed.

Those who have had multiple miscarriages.
The difficult experience of miscarriage often doesn’t have an identifiable cause. Sperm DNA damage has been correlated with pregnancy loss. DNA fragmentation index tests may give clues about the cause of recurrent pregnancy loss.

Those over 35 years old.
Sperm DNA fragmentation is more likely with age, and average level of damage increases about 33% between age 30 and age 50. Therefore, clients over 35 should consider more in-depth fertility testing like a sperm fragmentation test.

In the kit:

A sterile sample cup to easily collect your sample from home

The Legacy “vault,” a foam insulating structure to keep a stable temperature and prevent leaking during transportation

The transport media — a vial of pink fluid — to keep sample viable during shipping

A tamper-proof strip to securely seal the shipping box

Free overnight shipping to send your sample to our lab for analysis

In your report:

Results for key parameters of semen quality, including sperm count, motility, morphology, and DNA fragmentation index.

Personalized client dashboard that includes more than just the numbers — it will give you context on what those numbers really mean for you and your family-building plans.

Evidence-based recommendations for improving your fertility health, based on your lifestyle and your personal results.

Exclusive access to book a virtual appointment with one of our male fertility specialist medical providers, to discuss your results and possible next steps.

Downloadable semen analysis summary report to share with your doctor.

Employer and organizational benefits

Insurance coverage: Leading insurance plans cover a semen analysis kit and a full year of frozen sperm storage. Check your insurance eligibility.

HSA or FSA. If you have a flexible spending account (FSA) or health savings account (HSA) as part of your healthcare benefits, you may use those dollars to pay for, or reimburse yourself for, your Advanced Semen Analysis.

Other employer benefits: Legacy is in-network with leading fertility benefits programs that may be offered by your employer, including Carrot, Kindbody, Stork Club, Progyny, and WINFertility. Learn more.

Military benefits: We offer discounted or free access to Legacy’s at-home semen analysis and sperm freezing to many active-duty and veteran members of the US military, through partnerships with the following organizations.

For Naval Special Warfare: Military Family Building Coalition’s Tadpole Project

For US Army Special Forces: Green Beret Foundation

For all service members and veterans: The Operation Baby Foundation

For veterans: Veterans Advantage

Interest-free monthly payments

Break the cost of sperm DNA fragmentation testing into monthly payments, interest-free, with Affirm financing. Your rate will depend on your credit, terms of payment, and purchase price. Select Affirm at checkout to explore your options. Or, try a Splitit payment plan.


Payment methods accepted

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Advanced analysis with sperm DNA fragmentation testing

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Data-driven lifestyle recommendations

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Overnight shipping

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On call fertility advisor

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Safe, secure multi-site sperm storage available

What to expect


Schedule your kit delivery.

Use the online dashboard to choose your preferred delivery date. If you don’t choose a date, your kit will automatically be delivered as soon as possible.


Receive and open your kit.

The kit will contain a sterile sample cup, a vial of transport media, an ice pack, and various packaging materials. Discard the ice pack, but keep everything else.


Make a plan.

You should abstain from ejaculating for 2–5 days before using the kit, and you should only return your sample between Monday and Thursday.

If you’re not producing your sample right away, put the transport media into the refrigerator. Once in the fridge, it can be used up until the expiration date printed on the vial.


Register and prepare your kit.

Scan the QR code inside the shipping box with a smartphone camera or visit your dashboard to register your kit.

Ensure your transport media is at room temperature before producing your sample.


Produce your sample.

Wash your hands and genitals. Produce your sample by ejaculating directly into the sample cup, without using lubricant or saliva. You do not need to fill the cup.

Add the transport media to the sample cup and swirl gently to mix. Close the cup.


Package and return your kit.

Use the included packaging materials to package your sample in the original shipping box. Return the kit through one of our shipping partners using either at-home pickup or via a participating location.


Receive your report.

Your results will be available in your online dashboard within 48 hours of our lab receiving your sample. You can schedule a call with a fertility expert to review your results and receive guidance on next steps.


What key factors do we measure?

Our Advanced Semen Analysis includes all the key metrics of sperm health, including sperm DNA fragmentation.












DNA fragmentation index


Semen plays a crucial role in carrying sperm through the reproductive tract. Producing the right amount means giving sperm the best chance at reaching the egg for fertilization.


A healthy sperm count means you’re producing and ejaculating enough sperm. A higher number improves your chances of conception, naturally or via fertility treatments.


Sperm concentration is the number of sperm per milliliter of semen. This number helps us compare your sample to others.


Motility is the percentage of your sperm that are moving properly; in order to fertilize an egg, your sperm must be “swimming.” Many at-home sperm count tests don’t include this essential metric, one of the most important factors in your fertility.


A sperm’s structure affects its ability to reach and fertilize an egg. Morphology is the percentage of sperm that are normally shaped; abnormalities may indicate poor sperm development or genetic issues. Fun fact: most sperm are abnormally shaped. Morphology as low as 4–14% is totally normal.



Sperm DNA integrity is an important factor in your ability to create a healthy pregnancy. Age, illness, and lifestyle can cause sperm DNA damage, called DNA fragmentation. Unfortunately, sperm DNA fragmentation testing is not included as part of a routine semen analysis. Our kit is the only at-home test that analyzes sperm DNA fragmentation.

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Test the five key metrics of sperm health.

If you’re trying to conceive

If you want to understand your fertility

For semen testing after a vasectomy

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2 semen analyses, 1 STI Test Kit, 5-year cryostorage

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Protect your fertility on your timeline.

If you’re considering sperm freezing

If you’re undergoing fertility treatments

For military members before deployment

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3 semen analyses, 1 DNA fragmentation analysis, 1 STI Test Kit, lifetime cryostorage



Secure your options to pass on your legacy.

If you’re considering hormone replacement therapy

If you’re receiving cancer treatment

If you’re considering a vasectomy

Frequently asked questions about sperm DNA fragmentation testing

Sperm DNA fragmentation testing, also known as a sperm DFI test or DFI sperm test, is the most effective way to identify the DNA fragmentation index (DFI). The sperm DNA fragmentation index refers to the percentage of sperm in a semen sample that has fragmented DNA. A higher DNA fragmentation index means that a larger percentage of a person’s sperm contains genetic damage.

Generally, a DNA fragmentation index over 30-50% is considered high and may impact fertility. In some cases, a DNA fragmentation index of 15-30% may cause fertility issues, especially if other abnormal semen parameters are present (such as low sperm count or poor motility or morphology), or if the female partner is older.

Several different tests use different technologies to identify and quantify sperm DNA damage:

Sperm chromatin dispersion (SCD) testing. In this process, sperm are carefully denatured — or chemically degraded — and examined under a high-powered microscope to identify the “halos” formed by loops of intact DNA. (Sperm with damage will not produce a halo.) This is the type of testing used in Legacy’s Advanced Semen Analysis.

Single cell gel electrophoresis assay (SCGE), also known as the comet assay. In this process, the cell membrane around sperm is broken down. Fragmented strands of DNA form a “tail,” like that on a comet, the presence and size of which indicates DNA damage.

Sperm chromatin structure assay (SCSA). A sperm chromatin structure analysis is a process in which sperm cells are stained and placed in the path of a laser beam. The laser will cause the dye to emit fluorescent light of a certain color: green indicates sperm with non-detectable levels of fragmented DNA, while yellow and red indicate moderate to high levels of sperm DNA fragmentation.

Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. In this test, an enzyme that attaches to broken DNA strands is “tagged” with fluorescent dye and added to sperm cells. Under a laser light, the cells with DNA damage will emit a different color than those with intact DNA.

These tests are typically performed in a speciality lab. Even if you collect a semen sample at your fertility clinic, it will be shipped to the lab for sperm DNA fragmentation testing.

The Advanced Semen Analysis from Legacy is priced at $570.

Comparative advanced sperm testing at a traditional clinic or lab will cost about $800 — $250 for the semen analysis, and $550 for the additional sperm fragmentation test.

If your analysis reveals that your sample isn’t viable for freezing — for example, if no sperm was found in your sample — you have a few options. You can speak to one of our male fertility specialists to understand what may be affecting your fertility, as well as some next steps you may want to take. You also may want to re-test to confirm any abnormal results.

Don’t worry: if your sample is deemed not viable for storage by our lab, we’ll refund you for the storage plan you purchased as well as any unused services, such as additional testing that could not be performed.

Learn more about our refund policy.

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