


A clinically validated option for DNA fragmentation testing
Measures the percentage of sperm that contain fragmented or damaged genetic information, in addition to standard parameters (count, motility, morphology)
Our mail-in kit means you can test from home instead of the clinic "collection room" — it's faster, more comfortable, and no time off work required
HSA/FSA eligible
Legacy offers the only clinically validated at-home option for sperm DNA fragmentation testing.
Our research, forthcoming in the American Journal of Men's Health, demonstrates that Legacy's kit protects sperm integrity, and that transit within our kit does not impact sperm DNA fragmentation index or morphology.
DNA fragmentation testing is recommended for anyone who:
Importantly, even those with normal results on a standard semen analysis can have elevated DNA fragmentation, which could impact a couple's ability to get or stay pregnant.
An advanced analysis measures:
In addition, our Advanced Semen Analysis includes lifetime access to our Insights dashboard, including your Sperm Score.
Legacy is in-network with leading health insurance plans, fertility benefits providers, and the Department of Veterans Affairs. You may be able to test and freeze your sperm for up to 100% off, depending on your plan. Coverage is available through: Aetna, Cigna, Emblem, UnitedHealthcare, Optum VACCN, TriWest, Progyny, Carrot, Maven, and WIN Fertility.
Learn more about ways to save on sperm testing & freezing.
Semen plays a crucial role in carrying sperm through the reproductive tract. Producing the right amount means giving sperm the best chance at reaching the egg for fertilization.
Sperm concentration is the number of sperm per milliliter of semen, as measured by a computer-assisted semen analysis device. We can use your sperm concentration and your semen volume to determine your total sperm count.
Motility is the percentage of your sperm that are moving properly. In order to fertilize an egg, your sperm must be “swimming,” so motility is an important parameter for fertility.
Our report breaks down the number of sperm with progressive motility — moving forward or in large circles — vs. sperm that are swimming in tight circles, known as non-progressive motility.
This metric is a calculation that includes sperm volume, concentration, and motility.
Because this parameter represents multiple sperm quality metrics, it's the best number we have to help us understand overall fertility and chances of conceiving with or without medical intervention.
Morphology is the percentage of sperm that are normally shaped. A sperm’s structure affects its ability to reach and fertilize an egg, and abnormalities may indicate poor sperm development or genetic issues.
Fun fact: most sperm are abnormally shaped. Morphology as low as 4–14% is totally normal.
The integrity of the DNA carried by sperm is an important factor in your ability to create a healthy pregnancy. and child. Age, illness, and lifestyle factors can cause damage this genetic material, called DNA fragmentation. This test measures the percentage of your sperm that contain damaged or fragmented DNA.
Sperm DNA fragmentation testing is not included as part of a routine semen analysis, but even those with normal results on a standard analysis may have elevated DNA fragmentation.
Legacy offers the only clinically validated at-home option for DNA fragmentation testing.
Best for:

Semen volume
Sperm concentration & count
Motility
Morphology
Lifetime access to your Insights dashboard, including your Sperm Score
Best for:

1 year of storage for 1 sample (renewable)
1 collection kit
Best for:

5 years of storage for 2 samples (renewable)
2 collection kits
Pre-freeze STI test
Best for:

25 years of storage for 3 samples
3 collection kits
Pre-freeze STI test
Advanced fertility testing
It's a good idea to test your sperm before you start trying to conceive — although anytime is a good time. Our standard semen analysis tests for the main parameters of sperm health (count, concentration, motility, morphology, and semen volume). Sperm testing can identify factors that may make conception difficult, and can reduce the time to pregnancy.
If you are over 35, planning IUI or IVF, or have a history of pregnancy loss, we recommend semen analysis plus sperm DNA fragmentation, a test that measures percentage of sperm that contain damaged DNA.
Once your kit arrives at the lab, your sample will be opened and labeled. The lab scientists will replenish the sperm transport media to ensure the health of the sperm is maintained during analysis.
Next, the sample will be spun in a piece of lab equipment called a centrifuge to separate the sperm from the semen. Once sperm is concentrated, the lab scientists load a slide containing a portion of the sample into the computer-assisted semen analysis (CASA) system. This will report on sperm count, concentration, and motility (the percentage of sperm that are moving or "swimming"). Sperm morphology, the size and shape of sperm, is evaluated manually by an andrology scientist with a high-powered microscope.
If you choose to complete sperm DNA fragmentation analysis, that's performed using the Halosperm sperm chromatin dispersion technique. In this technique, a small portion of your sample is exposed to chemicals that denature, or separate, the strands of DNA in your sperm. This allows the lab scientists to assess which sperm contain broken or damaged DNA that could contribute to issues getting or staying pregnant.
Legacy’s at-home sperm analysis is a clinic-grade semen analysis without the clinic. It looks at all the key metrics of sperm health, including semen volume, sperm concentration, sperm motility, total motile sperm count, sperm morphology, and sperm DNA fragmentation.
Many at-home sperm testing options don’t look at sperm motility, morphology, or DNA fragmentation, which are important parameters. We’re also the only at-home sperm freezing option that includes a post-thaw analysis, in which we freeze and thaw a small portion of your sample to assess if it’s a good candidate for cryopreservation (sperm freezing).
Basic Semen Analysis looks at count/concentration and motility
Standard Semen Analysis looks at count/concentration, motility, and morphology
Advanced Semen Analysis looks at count/concentration, motility, morphology, and sperm DNA fragmentation
A sperm DNA fragmentation test analyzes what percentage of your sperm have damaged DNA. This damage can contribute to infertility, pregnancy loss, and poor health outcomes for your future children.
Together with a semen analysis, sperm DNA fragmentation testing provides a more complete picture of your reproductive health and your chances of conception.
Sperm DNA fragmentation is measured as part of our Advanced Semen Analysis.
No, we can assess sperm DNA fragmentation as part of the same kit that tests for the standard parameters of sperm health (count, concentration, motility, morphology, and volume).
The Legacy kit includes:
The Standard & Advanced Semen Analysis options also include lifetime access to our Insights dashboard, including your Sperm Score and a comparison of your metrics against thousands of clients your age.
Legacy’s semen analysis kit has been clinically validated. Our research team has compared the results of our kit to a conventional, in-person semen analysis and found no significant difference. There is likely to be a slight decline in sperm motility when using mail-in kits because of time spent in transit.
Legacy’s kit includes a sperm transport liquid that, when mixed with your semen sample, keeps sperm alive and healthy during shipment to our lab for testing.
From prep to results, it can take less than a week.
As soon as you order your kit, it will be express shipped to you. You should abstain from ejaculating for 2–5 days before using the kit. You should collect and return your sample on the same day, between Monday and Thursday. Each kit includes free overnight shipping back to our lab.
Your results will be available in your online dashboard within 48 hours of our lab receiving your sample. If you choose sperm freezing, your sample will be frozen immediately.
Yes, you can use frozen sperm for intrauterine insemination (IUI), in vitro fertilization (IVF), or intracytoplasmic sperm injection (ICSI). If you’re doing a fertility treatment cycle, having a healthy frozen sperm sample on hand can be an important back-up plan.