Once your kit arrives at the lab, your sample will be opened and labeled. The lab scientists will replenish the sperm transport media to ensure the health of the sperm is maintained during analysis.
Next, the sample will be spun in a piece of lab equipment called a centrifuge to separate the sperm from the semen. Once sperm is concentrated, the lab scientists load a slide containing a portion of the sample into the computer-assisted semen analysis (CASA) system. This will report on sperm count, concentration, and motility (the percentage of sperm that are moving or "swimming"). Sperm morphology, the size and shape of sperm, is evaluated manually by an andrology scientist with a high-powered microscope.
If you choose to complete sperm DNA fragmentation analysis, that's performed using the Halosperm sperm chromatin dispersion technique. In this technique, a small portion of your sample is exposed to chemicals that denature, or separate, the strands of DNA in your sperm. This allows the lab scientists to assess which sperm contain broken or damaged DNA that could contribute to issues getting or staying pregnant.